While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. After lysis of the cells, the mixture of 30 µL cell supernatant and 150 µL 30 µg/mL DTNB incubated for 25 min. Generate more data points without the need to dilute. Add either alarmaBlue HS or alarmaBlue reagent to microplate wells (see recommended volumes) 3. 0 out of 5 $ 12,499. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The staphyloxanthin intermediates present in collected supernatant were quantified by measuring at different wavelengths using multi-label reader (Spectramax M3, USA). • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderWe would like to show you a description here but the site won’t allow us. Total phenolic contents were calculated from the gallic acid standard curve. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 1. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. In the M2, M5 and Plus 384, the light. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10 The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 6: bioNova científica, s. As for each measurement, I. 1). Qualify the fluorescence performance of the SpectraMax . 304 signal level saturation. 1) EarlyTox Glutathione Assay (6. Photometric accuracy. reader in the visible range between 405–750 nm with NIST and NMI. Furthermore, to investigate the effects of nucleotides on the interaction, the assay was repeated in the presence of 5 mM. 316 grating motor driver faulty. Our Price: $8,635. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Choose cables that meet the requirement described in this appendix. For crystal violet. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 01) + 0. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. A single colony of bacterial isolates was inoculated in Marine Broth using a sterile inoculation loop. Sphere-like cells were subcultured into single-cell suspension, and cells of ≥90% viability were used for sphere formation studies. Multimode Readers Comparison Chart. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The isolates were categorized. Read mode. 7. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The resorufin product formed was monitored by fluorescence spectroscopy (excitation energy at 530 nm and emission energy at 590 nm) every 10 min in Spectramax M3 (Molecular Devices). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 306 plate air cal. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Software Manual – SoftMax Pro. Microplate Readers include the M3, M4, exception is noted. 005) Photometric PrecisionSpectraMax M2, SpectraMax M5, PLUS, PLUS 384, 190, 340PC, 340PC 384 micro-plate spectrophotometers and the VersaMax microplate reader. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. The intracellular ROS level was also cross-confirmed using the DCF-DA method with a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA,. Choose from a three- (M3), four- (M4), or SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Note: In this user guide, all references to SpectraMax Multi-Mode. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Detection modalities are shown in Table 1-1. gingivalis -treated BMDMs (MOI = 50), with or without pretreatment of OTTSP167 or Compound 50 for 2 h. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence,. Luciferase Detection Instrumentation Guide. . RP-3500 is highly selective for ATR with 30-fold selectivity over mammalian target of rapamycin (mTOR) and more than 2,000-fold selectivity over ataxia. We would like to show you a description here but the site won’t allow us. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. Detection modalities are shown in Table 1-1. spr SpectraMax Plus 384 SpectraTest ABS2 v1. For example, microplates containing different samp les can be read using the same orCell viability는 세포막 Integrity 또는. 0 (PLA) software (Stegmann Systems GmbH, Rodgau, Germany) to calculate relative. Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. . AquaMax® 2000. Detection cartridge modularity allows for easy addition of new read modes within minutes without. SpectraMax® Paradigm® Multi-Mode Detection Platform. Serum IL-8 level quantification Serum IL-8 levels were quantified in sera from patients after HSCT using an enzyme-linked immunosorbent assay (Cat. Wavelength Selection. Light Source: Xenon Flash Lamp (1 joule/flash) Detectors: 2 photomultiplier tubes. Hydrophobicity assayThese settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 더 많은. 4 kilograms). The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Molecular Devices SpectraMax® series microplate readers. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Immunofluorescence ASC antibody (1:200, Abcam, ab155970, UK) was used for immunofluorescence in P. Transfection with siRNA. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. However, limitations such as engraftment failure and time-consuming for establishing and expanding PDX models followed by testing drug efficacy, and inability to subject to. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. 3. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The team have compared the effects of antibiotics on bacterial viability and. M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. The software allows custom calculations and personalized reporting due to its analysis, management capabilities, and data acquisitions. 315 can't find zero order. a SpectraMax M3 microplate reader (Molecular Devices Inc. Spectral Range. Metrics used to measure sensitivity include signal-to-background ratio (S/B), signal-to-noise ratio (S/N), lower limit of detection (LLD) and Z’-factor 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 312 gain calibration failed. SoftMax Pro. Choose from a three- (M3), four- (M4), orThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The ACE2 activity assay was performed in black 96-well plates with 50 μM ACE2-specific fluorogenic peptide substrate (M-2660) in a final. Bioz Stars score: 96/100, based on 1 PubMed citations. Collection in December 2013 and storage until extraction of Malaysian the A. 21. 00 . From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 315 can't find zero order. A microplate reader as• FlexStation® 3 Multi-Mode Microplate Reader• SpectraMax® i3/i3x Multi-Mode Microplate Reader • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers• SpectraMax® Paradigm® Multi-Mode Microplate Reader 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader Biotek Synergy H1 Hybrid Microplate Reader. 4. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Multimode Reader Comparison Chart. Microplate Readers Biotek Synergy 2 Plate. Find many great new & used options and get the best deals for Replacement Bulb for Molecular Devices SpectraMax M3 Flash Lamp at the best online prices at eBay! Free shipping for many products! Skip to main content. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Page 22 SpectraMax L Luminescence Microplate Reader User Guide To manually change the aperture: 1. Microplate Readers Molecular Devices VersaMax Microplate Reader. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The drawing shows a standard microplate of 15 mm height covered with a lid. The plates were protected from light and incubated at room temperature for 8 min. D 1 1. To determine growth rate, the log phase of growth. 2. Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Published Dec 16, 2020 | Updated Dec 16, 2020. Find regional support. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Expanded wavelength coverage. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9SpectraMax® L (software version 5. The net area under the curve (AUC) was estimated by subtracting the AUC of the blank from the AUC of the reacted. 305 reference level saturation. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. . Description 1. 2. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 116 SpectraMax M5 and M5e Multi-Mode. Italic and boldface type are used for emphasis. 0nm increments. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellLuminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. e. 4. 190-1000 nm. . The ROS level was detected by a reactive oxygen species assay kit (Beyotime, China). Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. 8. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Cables. Select wells and then add or select a group or blank and assignA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. SpectraMax ® M2 Multi-Mode Microplate Reader. Molecular Devices Spectramax Plate Reader. The test was repeated three times. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. . When a SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. book Page 21 Wednesday, July 7, 2010 2:09 PM. Filterless readers provide consistent results with steady temperature regulation. 1. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Choose from a three- (M3), four- (M4), orFluorescence was measured using a SpectraMax M3 plate reader equipped with an excitation at 490 nm and an emission at 535 nm. For more sample throughput, the system canStakMax Handling Stacker Base Unit. Molecular Devices SpectraMax M3 User Manual Download Operation & user’s manual of Molecular Devices SpectraMax M3 Laboratory Equipment for Free or View it Online on All-Guides. 2 release. S. Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Our absorbance plate readers feature our PathCheck Sensor. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. SpectraMax Microplate Readers Multi-mode Readers Single-mode readers SoftMax Pro Software GxP Compliance Solutions SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. ® reader to the computer. Learn more about its features, specifications, and applications in this user. 1. 7°C and the bottom to 36. 4. XTplusC Texture Analyser Languages: French, English and Greek. SpectraMax Line of Readers Now Includes 14 Unique Configurations to Accommodate Wide Range of Customer Detection Needs MDS Analytical Technologies Launches Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate ReadersAutomated Functions Autofocus, autoLED intensity, auto-exposure Autofocus Method Image-based autofocus User-trained autofocus Laser autofocus (option)2. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. . SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Use the 2. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper Module. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 4. SpectraMax Gemini EM/XPS. The resulting absorbance values were plotted against time. SpectraMax Luminometer Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Instruments: Cary 630 FTIR, SpectraMax® M3 Multi-Mode Microplate Reader, AR 2000 and TA. Antiretroviral activities. Plant material. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. All procedures completely complied with the manufacturer’s instructions. Mohd. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $12,125. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentThe SpectraMax iD3 reader offers the sensitivity and wavelength scanning capability needed to perform intrinsic tryptophan fluorescence measurements. 2. Description 1. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader. spr SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 10. The bacterial colony was mixed with. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. An assay plate containing a 15-point standard curve was read on the SpectraMax. Learn more about its features, specifications, and applications in this user guide. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email. Offer Ends In: 5 Days 05: 02: 03. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax Mini. book Page 1 Wednesday, July 7, 2010 2:09 PMA SpectraMax ® M3 microplate reader luminometer was used to quantify the chemiluminescence from the reactions as relative luminescence units (RLUs) every four seconds for three minutes. 5. Description 1. , Sunnyvale, CA). book Page 1 Tuesday,. We would like to show you a description here but the site won’t allow us. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. The fluorescence intensity of the cells was detected using a SpectraMax M3 florescence microplate reader (Molecular Devices) with excitation and emission wavelengths of 485 and 535 nm, respectively. After that, 100 µL of TMB solution was added as the substrate and kept for 15 min at room temperature. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive dataPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. For assays where cells were pre-treated with ABAH or vehicle control, cells were incubated in. The chamber contains springs that position the cuvette in the proper alignment for a read. . M2. 0 out of 5Growth curves were read in a Spectramax M3 plate reader, at 37 °C with shaking at an optical density of 600 nm; reads were taken every 5 min. SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Fluorescent Polarization: Solid black plates, as above. 2. The ALP activity level was determined, relative to that of the control group, as. The TPC of the samples was calculated by plotting a standard curve with different concentrations of gallic acid (10, 30, 60, 100, and 200 mg/L). Male 8 pin mini Din to Female 9D. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. 0 mm hex key. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. SpectraMax® Aquamax. 0200-0729. Fluorescence of the samples were read using a SpectraMax M3 instrument with an excitation set to 355 nm and emission set to 460 nm. For standard curve values, an albumin standard (Thermo Fisher Scientific) dilution serious. 1. Learn how you can utilize microplate readers to drive landmark discoveries. 3: The cuvette chamber. Summary: Accurate and reproducible A260 measurements can be made easily in SpectraMax Microplate Spectrophotometers with awareness of the optical properties of microplate materials and attention to technique. Tumorsphere formation assay and limiting dilution assay. The following document is intended to demonstrate setup of this instrument. The guide also covers the software features, the specifications, and the accessories of the systems. DevicesThe SpectraMax M2 And M2e Systems Provide The Multi Detection Microplate Readers With Dualmonochromators, Dual-mode Cuvette Ports, With Top & Bottom Reading. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. SpectraMax M5/M5e/M4/M3/M2/M2e. 0200-7027. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. 303 unable to cal dark current. Fluorescence Microplate Reader Comparison Chart. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. SpectraMax i3x Multi-Mode Detection Platform. Other technologies such as imaging. Italic and boldface type are used for emphasis. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. Moving a SpectraMax Multi-Mode Microplate Reader. 9000-0756. Mitochondrial activity was expressed by the decrease in OD (450–660 nm), discounting the average of the ODs of the controls . 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. High sample correlation coefficients (R 2) make it easy to. 0. We would like to show you a description here but the site won’t allow us. Cells cultured in 96 well plate are washed with PBS or HBSS (phenol red free), incubated with ~5-10 uM. 2. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. Monochromator, tunable 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence. Data of selected analytes in the graphs below illustrate sample correlations between 96-well and 384-well format of 16 human plasma samples. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. SoftMaxProSoftwareUserGuide 2 5014177G ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevices,LLC(“Molecular Devices”)equipment,software,reagents. . U2OS cell viability (%) = [A cells with different treatments −A contro ]/[A cells with no treatments − A control ] × 100. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 4. The innovative and flexible design features. 2. SpectraMax Paradigm Multi-Mode Detection Platform User Guide Loading and Unloading Microplates To load or unload a microplate: 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. All the experiments were done in triplicate. Microplate Reader Shelf. Molecular Devices Spectramax M3 Mulitmode Microplate Reader is available from The Lab World Group. The SpectraMax® M4 Multi-Mode Microplate Reader dual monochromator optics allow the widest range of applications without the need to change filters and delivers single-mode performance in one multi-mode platform. Accuris SmartDrop. The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode. I don’t care what you do or where…The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a series of multi-detection, dual-monochromator, multi-mode instruments with a triple-mode cuvette port and 6 to 384-well microplate reading capabilities. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. No accessories are needed and SoftMax® Pro. The luminescent signal was proportional to the amount of GSH or GSSG present. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. 4. STAKMAX. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Multimode Reader Comparison Chart. 9000-0756. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. Description 1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Page 1 SpectraMax ® GEMINI EM Dual-Scanning Microplate Spectrofluorometer Operator’s Manual 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0088 Rev. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] i3x Multi-Mode Detection Platform Features User-configurable applications User-configurable detection modules expand the reader’s detection capabilities to. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Examples: “Press carefully to engage,” “ Do not. Wafi’s main research topic aims to set up a pharmacodynamic model comparing antibiotic activities against biofilms and examine in parallel their effects on viability and biofilm mass. It does not work as expected. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Recommended SpectraMax iD3/iD5 reader settings. ZERO BIAS - scores, article reviews, protocol conditions and moreInstruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. Absorbance was read at 570 nm using a SpectraMax M3. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Figure 2-23: Bottle holder To install the bottle holder: 1. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices.